Obatala® offers in vitro studies in 2D and 3D systems. Our cell culture system, ObaCell®, consists of setting up the systems with our proprietary human-derived hydrogel, ObaGel®. Customers can request in vitro studies be performed solely in 2D or 3D culture or in both of our cell culture systems. Our cell culture services include cell isolation and expansion in 2D or 3D systems from tissues that are internally available at Obatala (human adipose, bone marrow, bone, or placenta) or from human- or animal-derived tissues provided by the customer. In addition to the isolation and expansion in our different cell culture systems, Obatala can perform lineage specific differentiation of cells, staining of cells, and quantification of the staining if desired. Our models can also test small molecules such a MRNA, stress genes, and knocking-down genes, creating a wide variety of applications!

The Details

Cell Selection/Enrichment: Obatala offers magnetic bead-based selection/enrichment of cells. Customers can opt to select/enrich the desired cells using Obatala’s validated panel of surface markers or provide information on the desired markers they wish to use. 

Flow Cytometry: Obatala offers flow cytometry analysis for all in vitro or in vivo studies that involve cell culture in either 2D or 3D systems. Obatala’s expertise involves performing analyses for stromal vascular cells (SVFs) and adipose-derived stromal/stem cells (ASCs); thus, our customers can choose flow cytometry analysis following ASC or SVF cell surface marker panels or request their own desired markers for analysis. In addition, Obatala can offer antigen expression analysis for an additional fee. These services can be adapted to any cell type and specie of origin as required by Obatala’s clients.

Gene Expression: Obatala offers gene expression analysis for all in vitro or in vivo studies. Customers can choose to include analyses with polymerase chain reaction, DNA isolation, or RNA isolation analyses in their studies.

Microscopy: Obatala offers a variety of microscopy services that include phase contrast, fluorescent, or confocal microscopy. All of Obatala’s microscopes are equipped with cameras able to take microscopy pictures for qualitative or quantitative analyses.

Protein Expression: Obatala offers proteomic analysis by mass spectrometry of cells and tissue samples. Additionally, we offer targeted proteomic analysis focused on Adipokine and Cytokine expression.

Functionality: Obatala offers functionality endpoint analyses to customers studying different disease models. These include but are not limited to oxygen consumption rate, glucose uptake, lipolysis and cell mitochondrial activity.

Analysis of Data: Obatala offers analyses of the results of all endpoints selected by the customer. Alternatively, customers can select for only the raw data to be reported. Analyses of data include image analysis using different quantification software, such as Cell Profiler and Image J. If the customer selects for the analysis of the resulting data, the data will be presented in a final report prepared by the Obatala research team.