Optimize your science with ObaGel® Coating
ObaGel Coating® is a comprehensive human-derived cell basement membrane matrix that supports the culture and expansion of multiple cell types. ObaGel® Coating provides a superior culture surface compared to tissue culture plastic or fibronectin coatings. This extracellular matrix encourages high density cell attachment and faster doubling times of primary cell types such as ASCs and HUVECs.
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700 µg/ml protein
350 µg/ml protein
No Coating
ASC - Day 1
HUVECs - Day 7
Figure 1. ASC and HUVECs seeded on 2D XGel Coating. a. Adipose-derived stem/stromal cells (ASC) were seeded at 25,000 cells per well of a 24-well plate. These images were taken <24 hours post seeding and demonstrate an increase in the number of cells attached to the surface when the coating is present. b. Human umbilical vein endothelial cells (HUVEC) cultured on ObaGel® coating (700 ug/ml and 350 ug/ml) and tissue culture plastic, stained with Alexa Fluor Phalloidin and Hoechst 33342 on day 7.
ObaGel® ECM Properties
Human-derived protein surface coating
Provided at 700 ug/ml protein concentration
Enhances cell attachment and proliferation
Increases retention of cells over culture maintenance
Provides native ECM for attachment of human cell lines
Supports adipogenic, osteogenic, and chondrogenic differentiation
Supports expansion through several passages of primary cell types
Publications
2023